Abstract:
Traditional medicine is used throughout the world as it is heavily dependent on locally available
plant species and plant-based products and capitalizes on traditional wisdom-repository of
knowledge. Medicinal plants constitute an extremely important resource for the development of
the global pharmaceutical, cosmetics and fragrance industry. The supplementation of human
diet with herbs, containing especially high amounts of compounds capable of deactivating free
radicals, may have beneficial effects, throughout recorded history, spices and herbs have been
used for flavoring foods and beverages and for medicinal purposes.
In this study crude extract of Mimusops Kummel fruit was isolated by using 99% of n-hexane, chloroform
and methanol solvents. The percent yield of crude extracts for n-hexane, chloroform and methanol
extracts were 3.2%, 5.8%, and 8% respectively. The crude extracts were investigated for antibacterial
screening by agar well diffusion method against three Gram negative (Klebsiella pneumonia,
pseudomonas aeurginosa and Escherichia coli); one Gram positive (Staphylococcus aureus) bacteria.
Antibacterial activity of each crude extract was tested. Methanol extract of mimusopes kummel fruit has
great potency against antibacterial activity than n-hexane and chloroform extracts and methanol extract
has good inhibition zone relative to gentamicin positive control/standard. Phytochemical analysis was
determined for each crude extracts. Many phytochemicals were present in the fruit as it was checked by
chemical test especially in methanol extract. Glycosides, Steroids, Tannin, Alkaloids, Flavonoid,
Saponin, Quinones and Protein were present in methanol extract. The elucidation/structure determination
of separated compound from Mimusops Kummel methanol extract was not possible due to poor
spectroscopic information about the separated two major products. i.e the spectra of proton and carbon 13 NMR were less intense and in same range there were overlapped
