Isolation And Characterization Of Pigment Producing Fungus From Huluka Rivr Bank Soils And Optimization Of Conditions For Pigment Production

Abstract

Natural colors are becoming more popular since they have less of a negative impact on the environment and human health than chemically produced colorants. Due to the simplicity of handling the microorganisms and the production process, microbial pigments have been prioritized over all other naturally occurring coloring sources. The primary goal of this study was to isolate, characterize, and optimize the conditions for yellow pigment producing fungi from river bank soils of huluka river. From the six sample one potentially yellow color producing fungi (one) was isolated. After morphological feature characterization the isolated fungi was potentially proposed as Penisilium sps because of character it showed. Acidified ethanol demonstrated pigment extraction from varies organic solvents tested for the fungal isolate pigment extraction. Myo-insitol from the varies carbon source investigated showed optimum growth and pigment generation with OD of 3.0 while measured by spectophotometry. Amonium Nitrate, Yeast Extract and Beef extract were the three nitrogen source investigated to show optimum growth and pigment generation from the fungal isolate at OD of 3.0.And also the isolate pigment showed and pigment synthesis atPH-4, Ph-6, Ph-7 and ph-8 showed OD at 3.0. According to various incubation temperature the yellow pigment produced at room temperature and 28oC. This finding highlights the importance of selecting the right carbon source for optimal growth and pigment formation in microbial cultures. Myo-inositol is a key component in cell signaling pathways and has been shown to enhance cell growth and metabolism. By understanding the impact of different carbon sources on microbial cultures, researchers can optimize conditions for maximum productivity and efficiency. Overall, this study underscores the significance of choosing appropriate carbon sources, nitrogens source, optimum temprature, optimum Ph, optimum salt concentration and incubation period for achieving desired outcomes in microbial cultures. Further research into the effects of different nutrients on microbial growth could lead to advancements in biotechnology and industrial applications