Phytochemical, Antioxidant And Antimicrobial Properties Of (Botoro) Stereospermum Kunthianum , Leaf, Stem Bark And Root

Abstract

The aim of this thesis was to evaluate Phytochemical properties, antioxidant and antimicrobial activities of (Botoro) Stereospermum kunthianum , leaf,stem and root gathered from Bako tibe Woreda in West shoa zone, Oromia regional state western Ethiopia. The leaves , stem bark and roots of the plant were collected and dried under shade and processed to minimize its size and powdered by electrical mill, Powdered and sieved using 200micro meter sieve. The different parts of the plant extracted by different solvents Ethanol, Methanol, and ethyl acetate was prepared in order to study their antimicrobial applications against some pathogens. The ferrous tartrate method was used to measure a total phenolic content of tasted plant from its stem, bark, leaf, and roots. while the Dowd method, with some modifications, was used to determine the total flavonoid content. The total antioxidant capacity of the extracts was evaluated using the Phosphomolybdenum method. Staphylococcus aureus, Escherichia coli, S. epidermis, and Klebsiella pneumonia were obtained from Addis Ababa and two bacterial isolates Pseudomonas aeruginosa and Clinical isolated from in microbiology laboratory ambo University r. Different Aspergillus isolated from soils and yeasts candida albicans isolated from the sample was taken from the candiodiosis patients from Ambo General Hospital. In this study ethyl acetate leaf extract had the highest yield at 18 grams. From the results it was found that the methanol extract of tested plant had the greatest antimicrobial activity against pathogens within a 30mm inhibition zone. The root extract of ethanol was more effective against clinical isolate pathogen (30mm). And also the ethyl acetate extract of the stem bark was effective against clinical Isolate (30mm). The leaf's ethanol extract had a greater zone of inhibition against candida species. Ethanol extract of tasted plant had antifungal activity against Asper 1 (22 mm), and ethyl acetate extract of stem bark also had antifungal activity against Asper 2. The absolute enemy of oxidant content of leaf concentrate of tasted plant was higher than the remainder of the concentrate tried in which the OD was 1.206. In this study the, counter microbes, against candida and hostile to parasitic exercises of the metabolite extricated from various plant parts were assessed.