Abstract:
Mushrooms are widely known for producing a variety of metabolites that are
employed as functional foods, dietary supplements, and cosmetics as well as anti cancer, antibacterial, and antiaging agents.The main objective of this thesis was to
evaluate metabolite concentrations and antibacterial activities of certain wild and
cultivated mushrooms, as well as the efficacy of silver nanoparticles synthesized from
the wild mushroom culture filtrate. The mycelia of the different mushrooms were
grown on potato dextrose agar and malt extract agar. Total antioxidant, flavonoids,
and phenolic content were measured by using standard procedures. Using the well
diffusion method on Mueller Hinton agar, the antibacterial activity of metabolites
extracted from different mushrooms was evaluated. Silver nanoparticles were
synthesized by using the culture filtrate of three of the wild mushrooms and evaluated
against the different human pathogenic microorganisms and synthesized
nanoparticles were analyzed by FTIR. Data analysis was performed using SPSS
statistical software for Windows version 25 and Origin-pro 22. From the different
mushrooms evaluated for growth on different mycological media polypore PG 1 fully
colonize the petridishses within 72hrs of incubation. The fruiting bodies of wild
mushrooms had higher total antioxidants than the mycelia and the brown cup
mushrooms had the highest total antioxidant 1.3+0.11 OD. Antibacterial activity of
synthesized silver-nano particle which undergoes calcination showed activity against
the four bacterial pathogens and did not show activity against K.pnemonia and
clinical isolates and 25+1.22mm was inhibition zone observed against E.coli by
PG2. PAU4-based synthesized silver nano-particle (calcinated) showed activities
against all pathogens except P.aerogenesa and the highest inhibition zone was
against S.epidermidis (30+1.55mm). PG1, PG2 and PAU4 showed activities against
all pathogens tested except against S.epidermidis. The highest of all inhibition activity
was observed against E.coli by PAU4 which was 35+3.12. Overall, this study
showed that the presence of different metabolite contents and microbial activity of
the various tested cultivated and wild mushrooms, designates the possibility new and
effective antimicrobial drugs that will be effective against different microbial groups
including drug resistance ones.
