The Metabolite Concentrations And Antibacterial Activities Of Certain Wild And Cultivated Mushrooms, As Well As The Efficacy Of Silver Nanoparticles Synthesized From The Wild Mushroom Culture Filtrate

Abstract

Mushrooms are widely known for producing a variety of metabolites that are employed as functional foods, dietary supplements, and cosmetics as well as anti cancer, antibacterial, and antiaging agents.The main objective of this thesis was to evaluate metabolite concentrations and antibacterial activities of certain wild and cultivated mushrooms, as well as the efficacy of silver nanoparticles synthesized from the wild mushroom culture filtrate. The mycelia of the different mushrooms were grown on potato dextrose agar and malt extract agar. Total antioxidant, flavonoids, and phenolic content were measured by using standard procedures. Using the well diffusion method on Mueller Hinton agar, the antibacterial activity of metabolites extracted from different mushrooms was evaluated. Silver nanoparticles were synthesized by using the culture filtrate of three of the wild mushrooms and evaluated against the different human pathogenic microorganisms and synthesized nanoparticles were analyzed by FTIR. Data analysis was performed using SPSS statistical software for Windows version 25 and Origin-pro 22. From the different mushrooms evaluated for growth on different mycological media polypore PG 1 fully colonize the petridishses within 72hrs of incubation. The fruiting bodies of wild mushrooms had higher total antioxidants than the mycelia and the brown cup mushrooms had the highest total antioxidant 1.3+0.11 OD. Antibacterial activity of synthesized silver-nano particle which undergoes calcination showed activity against the four bacterial pathogens and did not show activity against K.pnemonia and clinical isolates and 25+1.22mm was inhibition zone observed against E.coli by PG2. PAU4-based synthesized silver nano-particle (calcinated) showed activities against all pathogens except P.aerogenesa and the highest inhibition zone was against S.epidermidis (30+1.55mm). PG1, PG2 and PAU4 showed activities against all pathogens tested except against S.epidermidis. The highest of all inhibition activity was observed against E.coli by PAU4 which was 35+3.12. Overall, this study showed that the presence of different metabolite contents and microbial activity of the various tested cultivated and wild mushrooms, designates the possibility new and effective antimicrobial drugs that will be effective against different microbial groups including drug resistance ones.