Phytochemical Investigation And Anti Bacterial Activity Of The Rhizome Of Drynaria Volkensii

Abstract

A medicinal plant is any plant in which one or more of its organs contains substances that can be used for the therapeutic purposes or which contain sources of ingredients that can be used in the synthesis and production of use full drugs. Drynaria Volkensii is one of the vascular epiphytic plant, families of polypodiaceae that is traditionally used to treat tooth ache. This research work is focused on phytochemical screening, structural elucidation and antibacterial activity of Drynaria Volkensii rhizomes extracts. Phytochemical screening of the rhizome of Drynaria volkensii extracts obtained from maceration and Soxhlet methods confirms the presence of many phytochemicals. The methanol, chloroform and hexane extract of the rhizome of Drynaria volkensii also tested for their anti-bacterial activity toward against staphylococcus aurous, Klebsiella pneumonia and Pseudomonas aurogenas. The methanol extracts obtained from maceration method showed good zone of inhibition against the three bacterial strains followed by methanol extracts obtained from Soxhlet methods. The chloroform extracts obtained from maceration and Hexane extracts obtained from Soxhlet methods undergo thin layer chromatography to set the sample for column chromatography and solvent selection. Finally 0.132g of chloroform extract and 0.39g of hexane extracts transported for spectroscopic purpose. Then the structure of the sample was proposed by using spectroscopic techniques including IR to identify the functional groups, 1H-NMR to determine number of proton, 13C-NMR to predict the number of all protonated of carbon and DEPT-135 to identify CH2 groups